| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
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| Autores: |
Emir,Ahmet
Emir,Ceren
Bozkurt,Buket
Onur,Mustafa Ali
Somer,Nehir Unver
Kaya,Gulen Irem
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Alkaloids
Amaryllidaceae
Plant extracts
High Performance Liquid Chromatography/method validation
High Perfomance Liquid Chromatography/quantitative analysis.
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| Resumo: |
ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100603
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902017000115063
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.53 n.1 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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